Rosner W, Polimeni S, Khan M S
Clin Chem. 1983 Jul;29(7):1389-91.
We isolated corticosteroid-binding globulin from human plasma and used the isolated protein as the antigen in raising antibodies in rabbits. The resulting antiserum was used to develop an RIA with which we can determine the globulin in as little as 0.007 microL of serum, although we routinely use, after appropriate dilution, 0.02 microL. The mean concentration of corticosteroid-binding globulin in the serum of 40 normal subjects was 32.4 (SD 4.2) mg/L. The correlation (r) between results with this RIA and those by an established binding assay was 0.94.
我们从人血浆中分离出皮质类固醇结合球蛋白，并将分离出的蛋白质作为抗原用于在兔子体内产生抗体。所得抗血清用于开发一种放射免疫分析方法，利用该方法我们能够测定低至0.007微升血清中的球蛋白，不过我们常规使用时，经过适当稀释后使用0.02微升。40名正常受试者血清中皮质类固醇结合球蛋白的平均浓度为32.4（标准差4.2）毫克/升。该放射免疫分析方法的结果与既定结合分析方法的结果之间的相关性（r）为0.94。
