Shiono T, Hayasaka S, Mizuno K
Exp Eye Res. 1983 Jun;36(6):871-6. doi: 10.1016/0014-4835(83)90040-4.
The effects of buffer concentrations, pH, and temperatures on the release of enzymes from lysosomes of the bovine retinal pigment epithelium were studied in vitro. Cathepsin D, arylsulfatase, and acid phosphatase were used as lysosomal marker enzymes. Elevation of temperature caused a marked increase in the release of cathepsin D and arylsulfatase from lysosomes, but little changes in the release of acid phosphatase. Acidic conditions accelerated the release of arylsulfatase and acid phosphatase. Different buffer concentrations had little effect on the release of these enzymes from lysosomes.
体外研究了缓冲液浓度、pH值和温度对牛视网膜色素上皮细胞溶酶体中酶释放的影响。组织蛋白酶D、芳基硫酸酯酶和酸性磷酸酶用作溶酶体标记酶。温度升高导致组织蛋白酶D和芳基硫酸酯酶从溶酶体中的释放显著增加，但酸性磷酸酶的释放变化不大。酸性条件加速了芳基硫酸酯酶和酸性磷酸酶的释放。不同的缓冲液浓度对这些酶从溶酶体中的释放影响不大。
