el-Hifnawi E, Kühnel W, el-Hifnawi A, Laqua H
Department of Ophthalmology, Medical University of Lübeck, Germany.
Ann Anat. 1994 Dec;176(6):505-13. doi: 10.1016/s0940-9602(11)80384-5.
Using ultrahistochemical and immunohistochemical techniques, localization of acid phosphatase and cathepsin D was demonstrated in the retina and pigment epithelium of 1 to 42 day old RCS rats and its nonaffected congenic rat strain. Both enzymes are present in the pigment epithelium of the normal and dystrophic rat eye. As early as the age of 1 week, it was found that the lysosomes in the dystrophic rat retina are less stable in releasing acid phosphatase than those of control animals. Infiltration of cathepsin D into the subretinal space could first be detected with certainty in 2-week-old animals. The fragility of the lysosomal membrane and, therefore, the release of both enzymes became more pronounced as the animals aged. The findings of this study indicate that the instability of the lysosomal membrane in the RCS rat pigment epithelium may initiate degeneration of photoreceptors and pigment epithelium. The demonstration of cathepsin D activity has proved very helpful in revealing the physiological or pathophysiological condition of retinal pigment epithelium.
运用超组织化学和免疫组织化学技术，在1至42日龄的RCS大鼠及其未受影响的同基因大鼠品系的视网膜和色素上皮中证实了酸性磷酸酶和组织蛋白酶D的定位。这两种酶均存在于正常和营养不良大鼠眼睛的色素上皮中。早在1周龄时就发现，营养不良大鼠视网膜中的溶酶体在释放酸性磷酸酶方面比对照动物的溶酶体更不稳定。在2周龄的动物中首次能够确切检测到组织蛋白酶D渗入视网膜下间隙。随着动物年龄的增长，溶酶体膜的脆性以及因此两种酶的释放变得更加明显。本研究结果表明，RCS大鼠色素上皮中溶酶体膜的不稳定性可能引发光感受器和色素上皮的退化。组织蛋白酶D活性的证实已被证明在揭示视网膜色素上皮的生理或病理生理状况方面非常有帮助。
