Zborowski J, Dygas A, Wojtczak L
FEBS Lett. 1983 Jun 27;157(1):179-82. doi: 10.1016/0014-5793(83)81141-7.
It is shown that the trypsin-treatment of rat liver mitochondria, depleted of the outer membrane, causes a strong inactivation of phosphatidylserine decarboxylase. This inactivation is dependent on trypsin concentration and the time of digestion in a similar manner as the inactivation of cytochrome oxidase. Under these conditions only a moderate inactivation of succinate dehydrogenase is observed. Phosphatidylserine decarboxylase is thus localized in the outer leaflet of the inner mitochondria membrane or, at least, is accessible from the outer surface of the inner membrane.
结果表明，用胰蛋白酶处理去除外膜的大鼠肝线粒体，会导致磷脂酰丝氨酸脱羧酶强烈失活。这种失活与胰蛋白酶浓度和消化时间有关，其方式与细胞色素氧化酶的失活相似。在这些条件下，仅观察到琥珀酸脱氢酶的适度失活。因此，磷脂酰丝氨酸脱羧酶定位于线粒体内膜的外小叶，或者至少可从内膜的外表面接触到。
