Hashimoto K, Suzuki Y, Kinoshita K, Takahashi G, Yasuhira K
J Chromatogr. 1983 May 6;260(2):429-38. doi: 10.1016/0021-9673(83)80050-8.
Microsomal metabolism of 3-methylcholanthrene (MC) was examined by computerized gas chromatography-mass spectrometry. Five mono-, four di- and thirteen trihydroxylated metabolites were found after incubation of MC in mouse liver microsomal fraction for 15 min, in the presence of NADPH. Among these metabolites, three mono- and three dihydroxylated metabolites were identified by means of authentic samples. The chemical structures of the other metabolites were deduced from their characteristic mass spectral fragmentations. This is the first description of trihydroxylated metabolites in MC metabolism in vitro and in vivo.
通过计算机化气相色谱 - 质谱法检测了3 - 甲基胆蒽（MC）的微粒体代谢。在存在NADPH的情况下，将MC在小鼠肝脏微粒体组分中孵育15分钟后，发现了5种单羟基、4种二羟基和13种三羟基化代谢物。在这些代谢物中，通过真实样品鉴定出3种单羟基和3种二羟基化代谢物。其他代谢物的化学结构通过其特征性质谱裂解推导得出。这是首次对MC体外和体内代谢中的三羟基化代谢物进行描述。
