Kinoshita K, Hashimoto K, Takahashi G, Yasuhira K
Gan. 1980 Apr;71(2):181-9.
Metabolites of 3-methylcholanthrene in tissues and excreta of rats and mice were extracted, directly or after removal from conjugation, with ethanol or ethyl acetate, purified by Sephadex LH-20 column chromatography, and examined by gas chromatography-mass spectrometric analysis. Data from the present experiments showed that the extracts after chromatographic purification were contaminated with some tissue-derived sterols such as cholesterol. As compounds related to 3-methylcholanthrene, a dehydro compound, 1- and 2-hydroxy compounds, 11-phenol and two other phenols were identified in the extracts. Some Ketones and cis-1,2-dihydroxy compounds were also detected on occasion. Some trihydroxy compounds were suggested. This is of interest because the trihydroxylated metabolites have been pointed out as precursors of ultimate carcinogen of MC recently. The high sensitivity of the lung to MC carcinogenesis was not interpreted only by specific distribution in tissue of metabolites of the hydrocarbon.
用乙醇或乙酸乙酯直接或在去除结合物后，提取大鼠和小鼠组织及排泄物中3-甲基胆蒽的代谢产物，通过葡聚糖凝胶LH-20柱色谱法进行纯化，并采用气相色谱-质谱分析进行检测。本实验数据表明，色谱纯化后的提取物被一些组织衍生的甾醇（如胆固醇）污染。作为与3-甲基胆蒽相关的化合物，在提取物中鉴定出一种脱氢化合物、1-和2-羟基化合物、11-苯酚以及另外两种苯酚。偶尔也检测到一些酮类和顺式-1,2-二羟基化合物。还推测出一些三羟基化合物。这一点很有意思，因为最近有人指出三羟基化代谢产物是MC最终致癌物的前体。肺部对MC致癌作用的高敏感性不能仅仅通过烃类代谢产物在组织中的特定分布来解释。
