Nissen H P, Schirren C, Heinze I, Kreysel H W
Andrologia. 1978 May-Jun;10(3):211-4.
Human semen contains beside the major carbohydrate fructose: galactose, glucose, sorbitol and inositol. By means of gas-liquid chromatography it is possible to identify also L-fucose. Our method requires the removal of hexosamines, amines and amino acids after hydrolysis. The gas-chromatographic separation of the carbohydrates is achieved in form of their trimethylsilyl derivatives. By this method the fucose content of several freeze dried human semen samples of different diagnosis could be determined.
