Kennedy L T, Hanbauer I
J Neurochem. 1983 Jul;41(1):172-8. doi: 10.1111/j.1471-4159.1983.tb13666.x.
In rat striatal membranes, NaCl induced a twofold increase in the maximal number of cocaine binding sites but did not alter the affinity of these sites for cocaine. This effect was concentration-dependent, specific to sodium ions, and occurred in membranes prepared from corpus striatum but not from other brain regions. Lesions with 6-hydroxydopamine but not with kainic acid eliminated the sodium-induced increase in binding and produced a decrease in the Bmax of binding measured in the presence of NaCl. The capacity of a series of drugs to interfere with Na+-dependent cocaine binding correlated well with their capacity to inhibit [3H]dopamine uptake into rat striatal synaptosomes. The present results suggest that Na+-dependent cocaine binding sites are localized presynaptically on dopaminergic nerve terminals in corpus striatum, and may be related to dopamine uptake sites.
在大鼠纹状体膜中，氯化钠使可卡因结合位点的最大数量增加了两倍，但并未改变这些位点对可卡因的亲和力。这种效应具有浓度依赖性，对钠离子具有特异性，且发生在从纹状体而非其他脑区制备的膜中。用6-羟基多巴胺而非 kainic 酸进行损伤消除了钠诱导的结合增加，并导致在存在氯化钠的情况下测得的结合Bmax降低。一系列药物干扰钠依赖性可卡因结合的能力与其抑制[3H]多巴胺摄取到大鼠纹状体突触体中的能力密切相关。目前的结果表明，钠依赖性可卡因结合位点位于纹状体中多巴胺能神经末梢的突触前，并且可能与多巴胺摄取位点有关。
