Kiribuchi M, Miura K, Tokuda S, Kaneda T
J Nutr Sci Vitaminol (Tokyo). 1983 Feb;29(1):35-43. doi: 10.3177/jnsv.29.35.
To identify the synergistic hypocholesterolemic substance found in soybean oil unsaponifiable matter, rats were fed diets containing various fractions of the unsaponifiable matter prepared by silicic acid column chromatography. The plasma cholesterol level of the group fed the alcohol fraction, which mainly consisted of triterpene alcohols, was significantly lower and the effect was synergistic with soysterol. So the effect of cycloartenol and 24-methylenecycloartanol, which are main constituents of triterpene alcohols in soybean oil unsaponifiable matter, was investigated. Both compounds were prepared from gamma-oryzanol (ferulate) and were added (0.05%) respectively to the experimental diet containing 0.5% cholesterol and 1% soysterol. It was observed that both cycloartenol and 24-methylenecycloartanol in combination with soysterol greatly reduced the plasma cholesterol and enhanced cholesterol excretion. This suggests that the hypocholesterolemic activity of dietary vegetable oils may account for not only their fatty acid compositions and sterol contents but also the synergistic hypocholesterolemic effect of triterpene alcohols.
为了鉴定大豆油不皂化物中发现的协同降胆固醇物质，给大鼠喂食含有通过硅酸柱色谱法制备的不皂化物不同组分的日粮。喂食主要由三萜醇组成的乙醇组分的那组大鼠的血浆胆固醇水平显著降低，且该效果与大豆甾醇具有协同作用。因此，对大豆油不皂化物中三萜醇的主要成分环阿屯醇和24-亚甲基环阿屯醇的作用进行了研究。这两种化合物均由γ-谷维素（阿魏酸酯）制备，并分别以0.05%的比例添加到含有0.5%胆固醇和1%大豆甾醇的实验日粮中。观察到环阿屯醇和24-亚甲基环阿屯醇与大豆甾醇联合使用可大大降低血浆胆固醇并增加胆固醇排泄。这表明膳食植物油的降胆固醇活性可能不仅与其脂肪酸组成和甾醇含量有关，还与三萜醇的协同降胆固醇作用有关。
