原核生物基因组DNA抑制哺乳动物细胞转化。
Procaryotic genomic DNA inhibits mammalian cell transformation.
作者信息
Yoder J I, Ganesan A T
出版信息
Mol Cell Biol. 1983 May;3(5):956-9. doi: 10.1128/mcb.3.5.956-959.1983.
Abstract
Ltk- mouse cells were transformed to thymidine kinase prototrophy in the presence of carrier DNAs isolated from different organisms. Procaryotic genomic and phage DNA was consistently less effective as a carrier than was eucaryotic DNA. Mixing experiments indicate that DNA of procaryotic origin inhibits mammalian cell transformation.
摘要
在存在从不同生物体分离的载体DNA的情况下，Ltk-小鼠细胞被转化为胸苷激酶原养型。原核基因组DNA和噬菌体DNA作为载体的效果始终不如真核DNA。混合实验表明，原核来源的DNA会抑制哺乳动物细胞的转化。
相似文献
1
Procaryotic genomic DNA inhibits mammalian cell transformation.原核生物基因组DNA抑制哺乳动物细胞转化。
Mol Cell Biol. 1983 May;3(5):956-9. doi: 10.1128/mcb.3.5.956-959.1983.
2
Transfer of genes to Chinese hamster ovary cells by DNA-mediated transformation.通过DNA介导的转化将基因转移到中国仓鼠卵巢细胞中。
Somatic Cell Genet. 1982 Jan;8(1):23-39. doi: 10.1007/BF01538648.
3
On procaryotic gene expression in eucaryotic systems.关于真核系统中的原核基因表达。
Hum Genet. 1980;54(3):289-302. doi: 10.1007/BF00291572.
4
5
Biological assay of prokaryotic genes in mouse cells following DNA mediated transformation.DNA介导转化后小鼠细胞中原核基因的生物学测定。
Mol Gen Genet. 1981;181(4):525-31. doi: 10.1007/BF00428747.
6
Transformation of mammalian cells with genes from prokaryotes and eukaryotes. 1979.
Biotechnology. 1992;24:444-52.
7
8
Isolation and preliminary characterization of the Chinese hamster thymidine kinase gene.中国仓鼠胸苷激酶基因的分离与初步鉴定。
Mol Cell Biol. 1983 Oct;3(10):1815-23. doi: 10.1128/mcb.3.10.1815-1823.1983.
9
10
[Effect of DNA on the growth and viability of transformed H group streptococci].
Mikrobiologiia. 1968 Sep-Oct;37(5):908-13.
引用本文的文献
1
Enhanced transformation of human cells by UV-irradiated pSV2 plasmids.紫外线照射的pSV2质粒对人类细胞的转化增强
Mol Cell Biol. 1984 Jun;4(6):1169-71. doi: 10.1128/mcb.4.6.1169-1171.1984.
2
Molecular cloning of the mouse ouabain-resistance gene.小鼠哇巴因抗性基因的分子克隆
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1489-93. doi: 10.1073/pnas.81.5.1489.
3
Efficient transfer of cloned DNA into human diploid cells: protoplast fusion in suspension.将克隆DNA高效导入人二倍体细胞：悬浮中的原生质体融合
Mol Cell Biol. 1984 Nov;4(11):2549-52. doi: 10.1128/mcb.4.11.2549-2552.1984.
4
Integration and expression of several molecular forms of Rous sarcoma virus DNA used for transfection of mouse cells.用于转染小鼠细胞的劳斯肉瘤病毒DNA几种分子形式的整合与表达。
Mol Cell Biol. 1984 Jul;4(7):1260-9. doi: 10.1128/mcb.4.7.1260-1269.1984.
本文引用的文献
1
Mechanisms and applications of DNA-mediated gene transfer in mammalian cells - a review.DNA介导的基因转移在哺乳动物细胞中的机制与应用——综述
Gene. 1981 Jun-Jul;14(1-2):1-10. doi: 10.1016/0378-1119(81)90143-8.
2
Mechanisms of DNA uptake by mammalian cells: fate of exogenously added DNA monitored by the use of fluorescent dyes.哺乳动物细胞摄取DNA的机制：利用荧光染料监测外源添加DNA的命运
Proc Natl Acad Sci U S A. 1982 Jan;79(2):422-6. doi: 10.1073/pnas.79.2.422.
3
Linkage and expression of foreign DNA in cultured animal cells.外源DNA在培养动物细胞中的连锁与表达
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:829-38. doi: 10.1101/sqb.1981.045.01.101.
4
Biological assay of prokaryotic genes in mouse cells following DNA mediated transformation.DNA介导转化后小鼠细胞中原核基因的生物学测定。
Mol Gen Genet. 1981;181(4):525-31. doi: 10.1007/BF00428747.
5
Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences.特定pBR322 DNA序列对猴细胞中SV40复制的抑制作用。
Nature. 1981 Sep 3;293(5827):79-81. doi: 10.1038/293079a0.
6
High efficiency transformation by direct microinjection of DNA into cultured mammalian cells.通过将DNA直接显微注射到培养的哺乳动物细胞中来进行高效转化。
Cell. 1980 Nov;22(2 Pt 2):479-88. doi: 10.1016/0092-8674(80)90358-x.
7
Genetic and physical linkage of exogenous sequences in transformed cells.转化细胞中外源序列的遗传和物理连锁
Cell. 1980 Nov;22(1 Pt 1):309-17. doi: 10.1016/0092-8674(80)90178-6.
8
Transformation of mammalian cells with an amplifiable dominant-acting gene.用可扩增的显性作用基因转化哺乳动物细胞。
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3567-70. doi: 10.1073/pnas.77.6.3567.
9
Isolation of the chicken thymidine kinase gene by plasmid rescue.通过质粒拯救分离鸡胸苷激酶基因。
Nature. 1980 May 22;285(5762):207-10. doi: 10.1038/285207a0.
10
A new technique for the assay of infectivity of human adenovirus 5 DNA.一种检测人腺病毒5型DNA感染性的新技术。
Virology. 1973 Apr;52(2):456-67. doi: 10.1016/0042-6822(73)90341-3.
Zotero 插件