An automated bichromatic measurement of serum immunoglobulins.

A new, rapid, discrete sampling and automated bichromatic procedure for the measurement of various soluble antigen-antibody complexes of immunoglobulins is described. The incorporation of polymeric substances and salts into the reaction mixture has greatly increased the sensitivity, shortened the reaction time and decreased the cost of the tests. Statistical analyses of data from this method have shown them to correlate well with values obtained by a radial immunodiffusion procedure. The automated procedure is simple and specific, it can be run at a rate of 90 specimens an hour.