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Size fractionation of Trypanosoma brucei DNA: localization of the 177-bp repeat satellite DNA and a variant surface glycoprotein gene in a mini-chromosomal DNA fraction.

作者信息

Sloof P, Menke H H, Caspers M P, Borst P

出版信息

Nucleic Acids Res. 1983 Jun 25;11(12):3889-901. doi: 10.1093/nar/11.12.3889.

DOI:10.1093/nar/11.12.3889
PMID:6866765
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326014/
Abstract

We have size-fractionated intact DNA from Trypanosoma brucei into a major large DNA fraction (greater than 350S) and minor middle-sized (60-250S) and small (less than 60S) DNA fractions. Large DNA contains the rRNA genes, the basic copy genes for several variant surface glycoproteins (VSGs), including one which lies near a telomer, and the expression-linked copies of the two VSG genes. The middle-sized DNA contains at least one VSG gene, but the hybridization of this fraction with probes for the conserved repetitive sequences that mark the edges of the transposed segments of VSG genes, suggests that it may contain many VSG genes. The 177-bp repeat satellite DNA is also exclusively found in this fraction.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2cd/326014/749c1fcf2450/nar00357-0055-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2cd/326014/9e1d84245e1f/nar00357-0052-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2cd/326014/12111bc20f21/nar00357-0053-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2cd/326014/749c1fcf2450/nar00357-0055-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2cd/326014/9e1d84245e1f/nar00357-0052-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2cd/326014/12111bc20f21/nar00357-0053-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2cd/326014/749c1fcf2450/nar00357-0055-a.jpg

相似文献

1
Size fractionation of Trypanosoma brucei DNA: localization of the 177-bp repeat satellite DNA and a variant surface glycoprotein gene in a mini-chromosomal DNA fraction.
Nucleic Acids Res. 1983 Jun 25;11(12):3889-901. doi: 10.1093/nar/11.12.3889.
2
Tandem repetition of the 5' mini-exon of variant surface glycoprotein genes: a multiple promoter for VSG gene transcription?变异表面糖蛋白基因5' 小外显子的串联重复：VSG基因转录的多个启动子？
Cell. 1983 Oct;34(3):891-900. doi: 10.1016/0092-8674(83)90546-9.
3
Activation of the genes for variant surface glycoproteins 117 and 118 in Trypanosoma brucei.布氏锥虫中变异表面糖蛋白117和118基因的激活。
J Mol Biol. 1983 Jun 5;166(4):537-56. doi: 10.1016/s0022-2836(83)80283-6.
4
Antigenic variation in Trypanosoma brucei analyzed by electrophoretic separation of chromosome-sized DNA molecules.通过染色体大小的DNA分子电泳分离分析布氏锥虫的抗原变异。
Cell. 1984 May;37(1):77-84. doi: 10.1016/0092-8674(84)90302-7.
5
(TAA)n within sequences flanking several intrachromosomal variant surface glycoprotein genes in Trypanosoma brucei.布氏锥虫中几个染色体内变异表面糖蛋白基因侧翼序列中的（TAA）n
Nucleic Acids Res. 1985 May 10;13(9):3161-77. doi: 10.1093/nar/13.9.3161.
6
The IsTat 1.3 VSG multigene family in Trypanosoma brucei: retention of the expression linked copy through multiple antigenic switches.布氏锥虫中的IsTat 1.3 VSG多基因家族：通过多次抗原转换保留表达相关拷贝
Nucleic Acids Res. 1984 Dec 11;12(23):9051-66. doi: 10.1093/nar/12.23.9051.
7
Characterization of the 1.35 kilobase DNA repeat unit containing the conserved 35 nucleotides at the 5'-termini of variable surface glycoprotein mRNAs in Trypanosoma brucei.布氏锥虫可变表面糖蛋白mRNA 5'末端含保守35个核苷酸的1.35千碱基DNA重复单元的特征分析
Nucleic Acids Res. 1984 Jun 25;12(12):4907-20. doi: 10.1093/nar/12.12.4907.
8
The transposition unit of variant surface glycoprotein gene 118 of Trypanosoma brucei. Presence of repeated elements at its border and absence of promoter-associated sequences.布氏锥虫可变表面糖蛋白基因118的转座单元。其边界处存在重复元件且缺乏启动子相关序列。
J Mol Biol. 1983 Jun 15;167(1):57-75. doi: 10.1016/s0022-2836(83)80034-5.
9
The 5' flanking sequence of a Trypanosoma brucei variable surface glycoprotein gene.布氏锥虫可变表面糖蛋白基因的5'侧翼序列。
Mol Biochem Parasitol. 1987 Jun;24(2):163-74. doi: 10.1016/0166-6851(87)90103-4.
10
Coordinate transcription of variant surface glycoprotein genes and an expression site associated gene family in Trypanosoma brucei.布氏锥虫中变异表面糖蛋白基因与一个表达位点相关基因家族的协同转录
Cell. 1985 Aug;42(1):173-82. doi: 10.1016/s0092-8674(85)80113-6.

引用本文的文献

1
Single nucleotide polymorphisms and copy-number variations in the Trypanosoma brucei repeat (TBR) sequence can be used to enhance amplification and genotyping of Trypanozoon strains.单核苷酸多态性和重复序列（TBR）中的拷贝数变异可用于增强锥虫属菌株的扩增和基因分型。
PLoS One. 2021 Oct 25;16(10):e0258711. doi: 10.1371/journal.pone.0258711. eCollection 2021.
2
Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases.采用 PCR 方法和纳米孔测序对马拉维牛体内锥虫进行分子鉴定：对人类和动物锥虫病控制的流行病学意义。
Parasite. 2020;27:46. doi: 10.1051/parasite/2020043. Epub 2020 Jul 20.
3

本文引用的文献

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8
The small chromosomes of Trypanosoma brucei involved in antigenic variation are constructed around repetitive palindromes.布氏锥虫中参与抗原变异的小染色体是围绕重复回文序列构建的。
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10
The telomeric GGGTTA repeats of Trypanosoma brucei contain the hypermodified base J in both strands.布氏锥虫的端粒GGGTTA重复序列在两条链中都含有高度修饰的碱基J。
Nucleic Acids Res. 1996 Jul 1;24(13):2476-82. doi: 10.1093/nar/24.13.2476.
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The isolation of plasmids containing DNA complementary to messenger RNA for variant surface glycoproteins of Trypanosoma brucei.含有与布氏锥虫可变表面糖蛋白信使核糖核酸互补的DNA的质粒的分离。
Gene. 1980 Mar;8(4):391-417. doi: 10.1016/0378-1119(80)90043-8.
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Genomic environment of the expression-linked extra copies of genes for surface antigens of Trypanosoma brucei resembles the end of a chromosome.布氏锥虫表面抗原基因表达相关额外拷贝的基因组环境类似于染色体末端。
Nature. 1982 Sep 30;299(5882):451-3. doi: 10.1038/299451a0.
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Genomic environment of T. brucei VSG genes: presence of a minichromosome.布氏锥虫VSG基因的基因组环境：微型染色体的存在
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Molecular drive: a cohesive mode of species evolution.分子驱动：物种进化的一种凝聚模式。
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On the DNA content and ploidy of trypanosomes.关于锥虫的DNA含量和倍性
Mol Biochem Parasitol. 1982 Jul;6(1):13-23. doi: 10.1016/0166-6851(82)90049-4.
9
Rapid evolution of genes coding for variant surface glycoproteins in trypanosomes.锥虫中编码可变表面糖蛋白的基因的快速进化。
Gene. 1982 Feb;17(2):197-211. doi: 10.1016/0378-1119(82)90073-7.
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