Katsuyama T, Spicer S S
Am J Anat. 1977 Apr;148(4):535-54. doi: 10.1002/aja.1001480409.
Regional differentiation of the plasma membrane and related structures of the exocrine pancreas has been studied ultrastructurally and cytochemically. Fixation with an osmium tetroxide-silver acetate solution produced abundant fine precipitates on the luminal and basal surface of the centroacinar but not the acinar cells. Staining with dialyzed iron (DI) revealed the heaviest concentration of anionic sites on the luminal plasma membrane of the acinar cells, including the surface of both the intercellular canaliculi and the main lumen. The reactive sites on the apical acinar plasmalemma appeared to consist of discrete globules. DI-reactivity of the lateral basal membranes was most prominent in the centroacinar cells and essentially absent in the acinar cells but was weak relative to that of the acinar-cell apical plasmalemma. The lamina lucida of the basement membrane of the duct stained with DI, but that of basement membrane under acinar cells did not. Sialidase digestion prior to DI staining abolished the staining of plasma membranes. These results indicate that duct epithelial cells, including most prominently the centroacinar cells, are chiefly responsible for electrolyte and fluid transport.
已通过超微结构和细胞化学方法研究了外分泌胰腺质膜及相关结构的区域分化。用四氧化锇-醋酸银溶液固定后，在中央腺泡细胞的管腔面和基底面产生了大量细小沉淀物，但腺泡细胞未出现此现象。用透析铁（DI）染色显示，腺泡细胞管腔质膜上阴离子位点的浓度最高，包括细胞间小管和主管腔的表面。腺泡顶端质膜上的反应位点似乎由离散的小球组成。外侧基膜的DI反应性在中央腺泡细胞中最为突出，在腺泡细胞中基本不存在，但相对于腺泡细胞顶端质膜较弱。导管基底膜的透明板被DI染色，但腺泡细胞下方基底膜的透明板未被染色。在DI染色前进行唾液酸酶消化可消除质膜的染色。这些结果表明，导管上皮细胞，尤其是中央腺泡细胞，主要负责电解质和液体的转运。
