Bozhko G Kh, Voloshin P V, Galushko E I
Ukr Biokhim Zh (1978). 1983;55(3):325-8.
Gel chromatography and electrophoresis in polyacrylamide gel show that urea in vitro induces aggregation of blood proteins, with their content unchanged. The aggregation does not depend on the fibrinogen available. Comparison of the data on the effect of urea, DS-Na and thermal denaturation on the protein aggregation suggests that the aggregation is induced by local conformation transformations in the structure of certain protein molecules. At the same time, the data obtained do not permit neglecting the role of denaturation in the aggregation.
凝胶过滤和聚丙烯酰胺凝胶电泳表明，体外尿素可诱导血液蛋白质聚集，但其含量不变。这种聚集不依赖于可用的纤维蛋白原。对尿素、十二烷基硫酸钠和热变性对蛋白质聚集影响的数据比较表明，聚集是由某些蛋白质分子结构中的局部构象转变诱导的。同时，所获得的数据不能忽视变性在聚集中的作用。
