Stitt B L, Mindich L
Virology. 1983 Jun;127(2):446-58. doi: 10.1016/0042-6822(83)90157-5.
Bacteriophage phi 6 has a lipid- and protein-containing membrane as its outer covering. Two phi 6-coded proteins are known to be required to produce enveloped phage particles: one is P9, the major phi 6 membrane structural protein, and the other is P12, a nonstructural protein without which membrane fails to assemble around phage nucleocapsids. A particle containing phospholipid, P9, and two minor phi 6 membrane proteins has been found in cells pulse labeled with protein precursors at late times after infection. The P9 particle can be chased into phage and is dependent on active P12 for its formation. Models are presented in which the role of the P9 particle in phi 6 membrane assembly is discussed.
噬菌体φ6的外层覆盖物是一层含有脂质和蛋白质的膜。已知产生包膜噬菌体颗粒需要两种由φ6编码的蛋白质：一种是P9，即主要的φ6膜结构蛋白；另一种是P12，一种非结构蛋白，没有它，膜就无法围绕噬菌体核衣壳组装。在感染后期用蛋白质前体进行脉冲标记的细胞中发现了一种含有磷脂、P9和两种次要的φ6膜蛋白的颗粒。P9颗粒可以被追踪到噬菌体中，并且其形成依赖于活性P12。文中提出了一些模型，讨论了P9颗粒在φ6膜组装中的作用。
