Krug H
Arch Geschwulstforsch. 1978;48(2):145-9.
By impulse cytophotometry (ICP) it is possible to get a DNA-distribution curve of nuclei in cell suspensions. The ICP curve directly represents the ploidy pattern of the investigated cell population and, more indirectly the proliferation intensity. For practical tumor diagnosis in cytology by ICP as a first step we must know, wether the ICP is able to detect differences between normal and tumor cells by simple criteria. Together with our clinical coworkers we had investigated cell suspensions from carcinomas of the cervix, ovary, breast, larynx, stomach and malignant melanomas. Approximately one half of these tumors were poly- or aneuploid. In the remaining diploid tumors some did not show signs of strong proliferation in the form of high 4c-peaks in the ICP; for these the chances of detecting by ICP in exfoliative material are low. This is in agreement with our results on vaginal smears. Here we had a good correlation between the mean 4c-height of the ICP curves and the Papanicolaou grading. But mass screening is not the main application of ICP because the security of Pa-grading is more sufficient. In individual problematic cytological cases with repeated investigation ICP is a very valuable diagnostic aid.
通过脉冲细胞光度术（ICP），可以获得细胞悬液中细胞核的DNA分布曲线。ICP曲线直接代表所研究细胞群体的倍体模式，更间接地代表增殖强度。对于通过ICP进行细胞学的实际肿瘤诊断，作为第一步，我们必须知道ICP是否能够通过简单标准检测正常细胞和肿瘤细胞之间的差异。我们与临床同事一起研究了来自子宫颈、卵巢、乳腺、喉、胃的癌以及恶性黑色素瘤的细胞悬液。这些肿瘤中约有一半是多倍体或非整倍体。在其余的二倍体肿瘤中，一些在ICP中没有以高4c峰的形式表现出强烈增殖的迹象；对于这些肿瘤，在脱落材料中通过ICP检测到的可能性较低。这与我们在阴道涂片上的结果一致。在这里，我们在ICP曲线的平均4c高度与巴氏分级之间有良好的相关性。但大规模筛查不是ICP的主要应用，因为巴氏分级的安全性更充分。在需要反复检查的个别有问题的细胞学病例中，ICP是一种非常有价值的诊断辅助手段。
