Krug H, Ebeling K
Exp Pathol (Jena). 1977;13(4-5):237-46. doi: 10.1016/s0014-4908(77)80008-2.
Tumor cell suspensions from 100 biopsies of squamous cell carcinomas of the cervix uteri were investigated by impulse cytophotometry (ICP) after staining with pepsin-ethidium bromide. We estimated the ploidy pattern by comparing the ICP curves with normal diploid material. The originally linearly classified curves were transformed in a logarithmical manner. We found 48 diploid populations and 42 polyploid populations, among them 19 tetraploid tumors. In these the 4c-peak was the highest. In 10 cases there were aneuploid stem lines, mainly lying between 2c and 4c; 2 curves had a hypertetraploid pattern. Helpful for interpretation of the whole curve is the introduction of an index: the "relative mean DNA-content" (DNA). In 15 diploid tumors we found a low proliferating rate, characterized by low 4c-peaks. For interpretation of polyploid cell cycles a completed nomenclature of cell cycle phases was introduced. Comparing our material with chromosome analyses in laterature we found more polyploid, e.g. tetraploid tumors. It can be assumed that the estimation of ploidy pattern solely by DNA measurements has another meaning than by chromosome analyses. The higher ploid peaks in DNA distribution curves (e.g. ICP-karyograms) represent not only true polyploid nuclei but also nuclei which are in a blocked premitotic resting phase (G2 for 4c; G'2 for 8c and so on). The ICP is a valuable method for estimating biological pecularities of tumor cell suspensions.
对取自100例子宫颈鳞状细胞癌活检组织的肿瘤细胞悬液，在用胃蛋白酶 - 溴化乙锭染色后，通过脉冲细胞光度法（ICP）进行研究。我们通过将ICP曲线与正常二倍体材料进行比较来估计倍体模式。最初线性分类的曲线以对数方式进行转换。我们发现48个二倍体群体和42个多倍体群体，其中19个四倍体肿瘤。在这些肿瘤中，4c峰最高。在10例中存在非整倍体干细胞系，主要位于2c和4c之间；2条曲线具有超四倍体模式。引入一个指数“相对平均DNA含量”（DNA）有助于解释整个曲线。在15个二倍体肿瘤中，我们发现增殖率较低，其特征是4c峰较低。为了解释多倍体细胞周期，引入了完整的细胞周期阶段命名法。将我们的材料与文献中的染色体分析进行比较，我们发现更多的多倍体，例如四倍体肿瘤。可以假设，仅通过DNA测量来估计倍体模式与通过染色体分析具有不同的意义。DNA分布曲线（例如ICP核型图）中较高的倍体峰不仅代表真正的多倍体核，还代表处于有丝分裂前静止期受阻的核（4c为G2期；8c为G'2期等）。ICP是估计肿瘤细胞悬液生物学特性的一种有价值的方法。
