Hughes H, Smith C V, Horning E C, Mitchell J R
Anal Biochem. 1983 Apr 15;130(2):431-6. doi: 10.1016/0003-2697(83)90612-7.
Peroxidation of membrane lipids has been implicated in the toxicity of reactive oxygen intermediates and of several hepatotoxins, but the specific products of this peroxidation in vivo have not been chemically identified. A method for the isolation, identification, and quantitation of specific lipid hydroperoxy and hydroxy acids formed in vivo has been developed. Hydroxylated derivatives of linoleic, arachidonic, and docosahexaenoic acids formed in mouse liver phosphatidylcholines following carbon tetrachloride administration were isolated by high-pressure liquid chromatography and identified as the trimethylsilyl ether methyl ester derivatives by gas chromatography-mass spectrometry. This methodology should be important for the investigation of the role of lipid peroxidation in a variety of normal physiologic and pathologic processes.
膜脂过氧化作用与活性氧中间体及几种肝毒素的毒性有关，但这种过氧化作用在体内的具体产物尚未经化学鉴定。现已开发出一种用于分离、鉴定和定量体内形成的特定脂质氢过氧化物和羟基酸的方法。在给予四氯化碳后，通过高压液相色谱法从小鼠肝脏磷脂酰胆碱中分离出在其中形成的亚油酸、花生四烯酸和二十二碳六烯酸的羟基化衍生物，并通过气相色谱 - 质谱法将其鉴定为三甲基硅醚甲酯衍生物。该方法对于研究脂质过氧化作用在各种正常生理和病理过程中的作用应该具有重要意义。
