Favier A, Maljournal B, Decoux G, Ruffieux D
Ann Biol Clin (Paris). 1983;41(1):45-50.
We describe a method of micro-assay of serum iron by atomic absorption without flame, after deproteinisation of the serum by molar hydrochloric acid. In this way, we can assay the serum iron in 10 microliters of serum by injection of the supernatant into a graphite oven. The results show a good correlation with those obtained by conventional techniques of atomic absorption and colorimetric assays using ferrozine and bathophenanthroline. However, great care must be taken in the cleaning of plastic test tubes.
我们描述了一种在血清用摩尔盐酸脱蛋白后,通过无火焰原子吸收法进行血清铁微量测定的方法。通过这种方式,我们可以将上清液注入石墨炉中,对10微升血清中的血清铁进行测定。结果表明,该方法与使用亚铁嗪和邻二氮菲的传统原子吸收和比色测定技术所获得的结果具有良好的相关性。然而,在清洗塑料试管时必须格外小心。