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通过核磁共振测量细胞内钾离子浓度

Measurement of intracellular potassium ion concentrations by n.m.r.

作者信息

Brophy P J, Hayer M K, Riddell F G

出版信息

Biochem J. 1983 Mar 15;210(3):961-3. doi: 10.1042/bj2100961.

Abstract

39K n.m.r. was used to detect and quantify K+ within human erythrocytes. A shift reagent consisting of an anionic complex of dysprosium(III) with sodium tripolyphosphate permitted a distinction to be made between K+ inside and outside erythrocytes. Intracellular K+ concentrations determined by this method were similar to values obtained by flame photometry.

摘要

利用39K核磁共振技术检测和定量人体红细胞内的钾离子。一种由镝(III)与三聚磷酸钠形成的阴离子配合物组成的位移试剂,能够区分红细胞内外的钾离子。用这种方法测定的细胞内钾离子浓度与火焰光度法测得的值相似。

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