通过23Na核磁共振测量人体红细胞主动排钠的最大速率。

23Na NMR measurement of the maximal rate of active sodium efflux from human red blood cells.

作者信息

Knubovets T L, Revazov A V, Sibeldina L A, Eichhoff U

机构信息

Institute of Chemical Physics, Academy of Science USSR, Moscow.

出版信息

Magn Reson Med. 1989 Feb;9(2):261-72. doi: 10.1002/mrm.1910090211.

DOI:10.1002/mrm.1910090211

PMID:2541305

Abstract

The method for 23Na NMR measurement of the maximal rate of active Na+ efflux from human red blood cells (RBC) is proposed. The nonpenetrating paramagnetic shift reagent (SR) bis(tripolyphosphate)dysprosium(III) complex is used to distinguish extracellular Na+ ions from intracellular. RBC are proved to retain their physiological activity in the presence of SR. Intracellular Na+ is shown to be 100% NMR visible. The levels of intracellular and extracellular Na+ and K+ ions are changed to decrease their concentration gradients across the erythrocyte membrane to make active Na+ efflux the only 23Na NMR measurable process; so the integrated areas of intra- and extracellular Na+ peaks remain invariant throughout the incubation period in the presence of 0.25 mM ouabain, a specific inhibitor of Na+, K+-ATPase. The accuracy of the proposed technique is evaluated to be 10%. The maximal Na+ efflux is determined to be 10.1 +/- 1.0 mM/h/liter of cells.

摘要

提出了一种用于测量人红细胞（RBC）中活性Na⁺外流最大速率的²³Na核磁共振（NMR）方法。使用非渗透性顺磁位移试剂（SR）双（三聚磷酸）镝（III）配合物来区分细胞外Na⁺离子和细胞内的Na⁺离子。已证明红细胞在存在SR的情况下能保持其生理活性。细胞内Na⁺被证明是100%可通过NMR检测到的。改变细胞内和细胞外Na⁺和K⁺离子的水平，以降低它们跨红细胞膜的浓度梯度，使活性Na⁺外流成为唯一可通过²³Na NMR测量的过程；因此，在存在0.25 mM哇巴因（一种Na⁺，K⁺-ATP酶的特异性抑制剂）的孵育期内，细胞内和细胞外Na⁺峰的积分面积保持不变。所提出技术的准确度评估为10%。确定最大Na⁺外流为10.1±1.0 mM/小时/升细胞。