半胱氨酸氧化对分离的肝细胞的影响。
The effect of cysteine oxidation on isolated hepatocytes.
作者信息
Viña J, Saez G T, Wiggins D, Roberts A F, Hems R, Krebs H A
出版信息
Biochem J. 1983 Apr 15;212(1):39-44. doi: 10.1042/bj2120039.
Abstract
Isolated hepatocytes incubated with 4mM-cysteine lose reduced glutathione, adenine nucleotides and intracellular enzymes, thus showing extensive membrane damage. The toxic effects of cysteine are enhanced by NH4Cl. Lactate, ethanol and unsaturated fatty acids afford significant protection against cysteine-induced cytoxicity. Addition of catalase to the incubation medium also protected against cysteine toxicity, indicating that H2O2 formed during the oxidation of cysteine is involved in the toxic effects observed. Under anaerobic conditions cysteine did not cause leakage of lactate dehydrogenase from cells, confirming that rapid autoxidation is an essential condition for development of the toxic effects of cysteine.
摘要
与4mM半胱氨酸一起孵育的分离肝细胞会失去还原型谷胱甘肽、腺嘌呤核苷酸和细胞内酶，从而显示出广泛的膜损伤。氯化铵会增强半胱氨酸的毒性作用。乳酸、乙醇和不饱和脂肪酸可有效保护细胞免受半胱氨酸诱导的细胞毒性。向孵育培养基中添加过氧化氢酶也可防止半胱氨酸毒性，这表明半胱氨酸氧化过程中形成的H2O2参与了所观察到的毒性作用。在厌氧条件下，半胱氨酸不会导致乳酸脱氢酶从细胞中泄漏，这证实了快速自氧化是半胱氨酸毒性作用发生的必要条件。
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