Branch D R, Muensch H A, Sy Siok Hian A L, Petz L D
Br J Haematol. 1983 Aug;54(4):573-8. doi: 10.1111/j.1365-2141.1983.tb02136.x.
We have investigated the effect of dithiothreitol (DTT) upon the Kell blood group system and other red cell antigens. All Kell blood group antigens studied (K, k, Kpa, Kpb, Jsa, Jsb and Ku) as well as the Cartwright (Yta) antigen were completely denatured after treatment with DTT. The Gerbich antigen was substantially weakened but not completely denatured. The Jsa and Jsb antigens appear to have an exquisite sensitivity to treatment with DTT and can be completely denatured using very low concentrations (less than or equal to 2 mM) whereas other Kell system antigens require much higher concentrations of DTT for their denaturation (100-200 mM). Of 38 other blood group antigens investigated, only the Yta antigen was completely denatured using 200 mM DTT. Furthermore, the Yta antigen was denatured within the same concentration range as Kell and one can speculate that this indicates some biochemical relationship between these two blood group systems. From our results, we conclude that: (1) at least two distinct disulfide (S-S) bonds are required for maintenance of the Kell blood group antigen system; (2) Jsa and Jsb antigens are distinctly different from other Kell system antigens based upon sensitivity to treatment with DTT; these antigens may be located on a different antigenic domain; and (3) the Yta antigen requires at least one disulfide bond for its maintenance of antigen integrity. Although the Gerbich antigen was not completely denatured, results indicate that disulfide bonds may also be important structural determinants for these antigens.
我们研究了二硫苏糖醇（DTT）对凯尔血型系统及其他红细胞抗原的影响。所有研究的凯尔血型系统抗原（K、k、Kpa、Kpb、Jsa、Jsb和Ku）以及卡特赖特（Yta）抗原在用DTT处理后均完全变性。盖尔比希抗原显著减弱但未完全变性。Jsa和Jsb抗原似乎对DTT处理极为敏感，使用极低浓度（小于或等于2 mM）即可完全变性，而其他凯尔系统抗原则需要更高浓度的DTT才能变性（100 - 200 mM）。在研究的其他38种血型抗原中，只有Yta抗原在使用200 mM DTT时完全变性。此外，Yta抗原在与凯尔抗原相同的浓度范围内变性，由此可以推测这表明这两个血型系统之间存在某种生化关系。根据我们的结果，我们得出以下结论：（1）维持凯尔血型抗原系统至少需要两个不同的二硫键（S-S）；（2）基于对DTT处理的敏感性，Jsa和Jsb抗原与其他凯尔系统抗原明显不同；这些抗原可能位于不同的抗原结构域；（3）Yta抗原维持抗原完整性至少需要一个二硫键。虽然盖尔比希抗原未完全变性，但结果表明二硫键可能也是这些抗原重要的结构决定因素。
