Singh A K, Capone R J, O'Shea P, Karlson K E
Circulation. 1983 Sep;68(3 Pt 2):II112-6.
We have studied the effects of cardioplegic solution (CS) on angiographic and histologic anatomy of bilateral vein grafts interposed into the carotid arteries of 10 dogs for up to 1 year. One vein (exp) was infused with cardioplegic solution (pH 7.7, K + 25 mEq/l, temperature 12 degrees C) every 15 min four times at pressures up to 80 mm and then interposed into the carotid system. The other vein (con) was kept in cold Ringer's lactate (12 degrees C) before interposition. Six weeks and up to 1 year after bypass, angiograms showed 80% graft patency of exp and con grafts. Immediate histologic examination showed no detectable differences among veins before and after perfusion and con veins. Histologic examination at 1 year showed enlargement of the grafts, smooth muscle hyperplasia, and increase in collagen, as well as disorientation of muscle fibers; cytoplasmic vacuolation occurred with equal frequency in both exp and con veins. Perfusion of the vein segment with cardioplegic solution did not appear to be detrimental to vein patency or histologic appearance so that this can be considered a safe method for delivery of cardioplegia in jeopardized ischemic myocardium at the time of bypass surgery.
我们研究了心脏停搏液（CS）对10只犬双侧颈内动脉静脉移植物血管造影及组织解剖结构的影响，观察时间长达1年。每隔15分钟，以高达80毫米汞柱的压力，向一根静脉（实验组）输注4次心脏停搏液（pH 7.7，钾离子浓度25毫当量/升，温度12摄氏度），随后将其植入颈动脉系统。另一根静脉（对照组）在植入前置于冷乳酸林格液（12摄氏度）中。搭桥术后6周及长达1年时，血管造影显示实验组和对照组移植物的通畅率均为80%。即时组织学检查显示，灌注前后的静脉及对照静脉之间未发现可检测到的差异。1年时的组织学检查显示移植物增大、平滑肌增生、胶原蛋白增加、肌纤维排列紊乱；实验组和对照组静脉中均同等频率地出现细胞质空泡化。用心脏停搏液灌注静脉段似乎对静脉通畅性或组织学外观并无损害，因此在搭桥手术时，这可被视为一种向濒危缺血心肌输送心脏停搏液的安全方法。
