Hoeg J M, Papadopoulos N M, Gregg R E, Brewer H B
Clin Chem. 1983 Aug;29(8):1459-62.
Previous evaluations of plasma lipoproteins from patients with type IIa hyperlipoproteinemia have demonstrated quantitative but not qualitative differences from lipoproteins isolated from normal subjects. Using a reproducible, sensitive, high-resolution agarose gel electrophoretic system, we evaluated the plasma lipoprotein of 22 consecutive, untreated, unrelated patients with type IIa hyperlipoproteinemia. Five distinct electrophoretic patterns were observed. This electrophoretic heterogeneity in type IIa hyperlipoproteinemia reflects subtle charge differences in the lipoproteins from these patients, which were not detected with paper electrophoresis or with lipoprotein quantification. There was little, if any, difference in total plasma cholesterol, low- or very-low-density lipoproteins, plasma triglycerides, or concentrations of apolipoproteins A-I, A-II, B, C-II, or E in these subgroups. However, unique clinical features were correlated with the electrophoretic patterns. These findings support the concept that the type IIa hyperlipoproteinemic phenotype includes several molecular defects that are reflected in subtle differences in lipoprotein charge.
先前对IIa型高脂蛋白血症患者血浆脂蛋白的评估表明，其与从正常受试者分离出的脂蛋白存在数量上而非质量上的差异。我们使用可重复、灵敏、高分辨率的琼脂糖凝胶电泳系统，对22例连续的、未经治疗的、无亲缘关系的IIa型高脂蛋白血症患者的血浆脂蛋白进行了评估。观察到五种不同的电泳模式。IIa型高脂蛋白血症中的这种电泳异质性反映了这些患者脂蛋白中细微的电荷差异，而纸电泳或脂蛋白定量分析未检测到这些差异。这些亚组在总血浆胆固醇、低密度或极低密度脂蛋白、血浆甘油三酯或载脂蛋白A-I、A-II、B、C-II或E的浓度方面几乎没有差异。然而，独特的临床特征与电泳模式相关。这些发现支持了这样一种观点，即IIa型高脂蛋白血症表型包括几种分子缺陷，这些缺陷反映在脂蛋白电荷的细微差异中。
