卤虫（卤虫）无节幼体作为一种致畸试验系统。

Brine shrimp (Artemia salina) nauplii as a teratogen test system.

作者信息

Kerster H W, Schaeffer D J

出版信息

Ecotoxicol Environ Saf. 1983 Jun;7(3):342-9. doi: 10.1016/0147-6513(83)90079-9.

Abstract

Brine shrimp increase in length rapidly after hatching. A teratogen test system is based on disruption of elongation between 24 and 48 hr after wetting of the cysts. Teratogenicity of substances dissolved in the medium is assayed by comparison of average lengths of animals raised for the test period in suspect solution with average lengths of controls. The system is fast, inexpensive, and requires little skill. Brine shrimp are suited to testing industrial wastes, chemical formulations, drugs, and food additives that can be dissolved in water at 25 degrees C. The method appears unsuited to testing the teratogenicity of gases, particulates, very dilute wastes, or natural waters. Cadmium, mercury, lead, zinc, bromoform, n-butylphthalate, 1,2-dichloroethane, nitrobenzene, tetrachloroethylene, toluene, 1,2,4-trichlorobenzene, and 1,1,3-trichloroethane were found teratogenic. Chromium (III), chromium (VI), copper, chlorobenzene, chloroform, dimethyl sulfoxide (DMSO), and phenol were found not teratogenic. Other aquatic organism teratogen test systems are surveyed.

摘要

卤虫孵化后体长迅速增加。致畸试验系统基于卤虫卵浸湿后24至48小时内伸长过程的中断。通过比较在可疑溶液中饲养的受试动物的平均体长与对照动物的平均体长，来测定溶解在培养基中的物质的致畸性。该系统快速、廉价且所需技能少。卤虫适合测试可在25摄氏度下溶解于水的工业废物、化学制剂、药物和食品添加剂。该方法似乎不适用于测试气体、颗粒物、极稀废物或天然水的致畸性。已发现镉、汞、铅、锌、溴仿、邻苯二甲酸正丁酯、1,2 - 二氯乙烷、硝基苯、四氯乙烯、甲苯、1,2,4 - 三氯苯和1,1,3 - 三氯乙烷具有致畸性。已发现铬（III）、铬（VI）、铜、氯苯、氯仿、二甲基亚砜（DMSO）和苯酚无致畸性。还对其他水生生物致畸试验系统进行了综述。