Tanswell A K, Joneja M G, Lindsay J, Vreeken E
Exp Lung Res. 1983 Jul;5(1):37-48. doi: 10.3109/01902148309061503.
In vitro observations of fetal lung maturation are complicated by continued epithelial cell maturation in culture. Removal of trace quantities of cortisol from the culture system has been reported elsewhere by Torday to prevent epithelial cell differentiation, but also prevent cell growth. In this report we have developed monolayer cell cultures from 18-, 19-, 20-, and 22-day-gestation fetal rat lung in a culture system stripped of steroid and thyroid hormones. These cultures demonstrate lamellar body development comparable to that seen in vivo at the gestational age at which cultures were developed; the gestation-dependent increase in precursor incorporation into saturated lecithin observed in these cultures is similar to that reported in in vivo studies, yet cell growth in culture has been preserved.
体外观察胎儿肺成熟会因培养过程中上皮细胞持续成熟而变得复杂。托迪在其他地方报道，从培养系统中去除微量皮质醇可防止上皮细胞分化，但也会阻止细胞生长。在本报告中，我们在去除类固醇和甲状腺激素的培养系统中，从妊娠18天、19天、20天和22天的胎鼠肺中培养出单层细胞。这些培养物显示出板层小体的发育情况与在培养物所对应的胎龄时体内观察到的情况相当；在这些培养物中观察到的前体掺入饱和卵磷脂的情况与体内研究报道的情况相似，且培养物中的细胞生长得以保留。
