Tanswell A K, Joneja M G, Vreeken E, Lindsay J
Exp Lung Res. 1983 Jul;5(1):49-60. doi: 10.3109/01902148309061504.
Differentiation-arrested and hormone-depleted monolayer cultures were developed from rat fetal lungs of 18-, 19-, 20-, and 22-days gestation. Incorporation of [3H]-choline into saturated phosphatidylcholine increased, whereas the rate of cell division decreased, with advancing gestational age. Both functions were modified by physiological concentrations of glucocorticoids, thyroid hormones, and insulin. Dexamethasone (0.055-5.5 nM) increased [3H]-choline incorporation into total saturated phosphatidylcholine in immature cultures only, but caused secretion in mature (day-22) cultures. Triiodothyronine (0.055-5.5 nM) increased [3H]-choline incorporation into total and secreted saturated phosphatidylcholine at all gestational ages. Insulin (5-50 microU/ml) inhibition of [3H]-choline incorporation into saturated phosphatidylcholine was evident only in mature cultures. Dexamethasone (0.55 nM), triiodothyronine (5.5 nM), and insulin (50 microU/ml) also had gestation-dependent effects on cell division.
从妊娠18天、19天、20天和22天的大鼠胎肺中培养出分化停滞且激素缺乏的单层培养物。随着胎龄增加，[3H] - 胆碱掺入饱和磷脂酰胆碱的量增加，而细胞分裂速率降低。糖皮质激素、甲状腺激素和胰岛素的生理浓度均改变了这两种功能。地塞米松（0.055 - 5.5 nM）仅在未成熟培养物中增加了[3H] - 胆碱掺入总饱和磷脂酰胆碱的量，但在成熟（第22天）培养物中导致分泌。三碘甲状腺原氨酸（0.055 - 5.5 nM）在所有胎龄时均增加了[3H] - 胆碱掺入总饱和磷脂酰胆碱和分泌饱和磷脂酰胆碱的量。胰岛素（5 - 50微单位/毫升）对[3H] - 胆碱掺入饱和磷脂酰胆碱的抑制作用仅在成熟培养物中明显。地塞米松（0.55 nM）、三碘甲状腺原氨酸（5.5 nM）和胰岛素（50微单位/毫升）对细胞分裂也有依赖于胎龄的影响。
