Sigdestad C P, Grdina D J, Ando K
Experientia. 1979 Jun 15;35(6):815-7. doi: 10.1007/BF01968269.
Cells from a murine fibrosarcome (FSa) have been grown in vitro as multicell tumor spheroids (MTS). The growth rate of these MTS was determined. Following selected periods of growth, MTS were made into a single cell suspension and separated on linear density gradients of Renografin. While only 1 population of cells were separated from small spheriods (400 mum diameter), at least 3 subpopulations of tumor cells were separated and isolated from large spheroids (800 mum in diameter).
来自小鼠纤维肉瘤（FSa）的细胞已在体外培养成多细胞肿瘤球体（MTS）。测定了这些MTS的生长速率。在选定的生长时间段后，将MTS制成单细胞悬液，并在泛影葡胺线性密度梯度上进行分离。从小球体（直径400μm）中仅分离出1个细胞群体，而从大球体（直径800μm）中至少分离并分离出3个肿瘤细胞亚群。
