Barrera C R
J Bacteriol. 1983 Aug;155(2):886-95. doi: 10.1128/jb.155.2.886-895.1983.
The formation of arthrospores in Mucor rouxii was studied by transmission and scanning electron microscopy and light microscopy. The arthrospores formed in a random manner in terminal and internal regions of the hyphae. The earliest appearance of the arthrospores was seen by scanning electron microscopy as compartments delineated by double ridges. These ridges probably corresponded to the site of septal wall formation. The elongated compartments varied considerably in size. As the arthrospores matured, they tended to separate as a result of a gradual change in the shape of the arthrospores to a nearly spherical form and also as the result of simultaneous degradation of the outermost cell wall layer. The mature arthrospores were surrounded by a complex cell wall consisting of at least three distinct layers in addition to the original hyphal cell wall. Crystal-like structures were seen in the cytoplasm of some of the arthrospores in addition to the usual organelles such as mitochondria, nuclei, and ribosomes. Septum formation by centripetal cell wall growth from the lateral hyphal wall was documented by transmission electron microscopy. However, evidence was also found which suggested that not all internal cell wall development in the fungal hyphae during arthrosporogenesis necessarily led to the formation of mature arthrospores.
通过透射电子显微镜、扫描电子显微镜和光学显微镜对鲁氏毛霉中节孢子的形成进行了研究。节孢子在菌丝的末端和内部区域随机形成。扫描电子显微镜观察到节孢子最早的形态是由双嵴界定的间隔区。这些嵴可能对应于隔壁形成的部位。拉长的间隔区大小差异很大。随着节孢子的成熟，由于节孢子形状逐渐变为近球形，以及最外层细胞壁层同时降解，它们倾向于分离。成熟的节孢子除了原来的菌丝细胞壁外，还被一层由至少三层不同层组成的复杂细胞壁所包围。除了线粒体、细胞核和核糖体等常见细胞器外，在一些节孢子的细胞质中还可见到晶体状结构。透射电子显微镜记录了从菌丝侧壁向心生长形成隔膜的过程。然而，也发现有证据表明，在节孢子形成过程中，真菌菌丝内并非所有的内部细胞壁发育都必然导致成熟节孢子的形成。
