通过二异丙醚 - 1 - 丁醇萃取法去除脂质后的血清中神经节苷脂的保留情况。

Retention of gangliosides in serum delipidated by diisopropyl ether-1-butanol extraction.

作者信息

Wong C G, Ladisch S

出版信息

J Lipid Res. 1983 May;24(5):666-9.

Abstract

Extraction with diisopropyl ether-1-butanol is a rapid method for the delipidation of serum without protein denaturation. We sought to confirm that this solvent system would also extract the highly polar acidic glycosphingolipids, gangliosides. In fact, however, both endogenous serum gangliosides and radiolabeled rat brain gangliosides added to serum were nearly quantitatively retained (87.5% and 98.7%, respectively) in the aqueous phase after two extractions. Therefore, while useful for the extraction of most lipids, this delipidation procedure cannot be used to remove gangliosides from serum or plasma.

摘要

用二异丙醚 - 1 - 丁醇萃取是一种快速的血清脱脂方法，不会使蛋白质变性。我们试图确认这种溶剂体系是否也能萃取高极性酸性糖鞘脂——神经节苷脂。然而，事实上，经过两次萃取后，内源性血清神经节苷脂以及添加到血清中的放射性标记大鼠脑神经节苷脂几乎都定量保留在了水相中（分别为87.5%和98.7%）。因此，虽然这种脱脂方法对大多数脂质的萃取很有用，但不能用于从血清或血浆中去除神经节苷脂。