Kantor H L, Prestegard J H
Biochemistry. 1978 Aug 22;17(17):3592-7. doi: 10.1021/bi00610a027.
The transformation of DMPC vesicles containing myristic acid to larger structures was examined by nuclear magnetic resonance techniques. The process was found to be characterized by a rate which is approximately first order in vesicle concentration. Rates, however, show a dramatic increase with increasing myristic acid content. The fatty acid is characterized as being active under conditions in which it is neutral and dispersed in the bulk bilayer phase. In addition a 1:1 correlation was noted between leakage of contents and vesicle transformation. On the basis of these observations a mechanism is postulated involving stabilization of leaks in bilayer membranes by myristic acid, and the resultant formation of activated sites for vesicle transformation. The relation to fusion in natural systems is discussed.
通过核磁共振技术研究了含有肉豆蔻酸的二肉豆蔻酰磷脂酰胆碱（DMPC）囊泡向更大结构的转变。发现该过程的特征在于其速率在囊泡浓度方面近似为一级反应。然而，随着肉豆蔻酸含量的增加，速率显著增加。该脂肪酸的特征是在其呈中性并分散在本体双层相的条件下具有活性。此外，还注意到内容物泄漏与囊泡转变之间存在1:1的相关性。基于这些观察结果，推测了一种机制，该机制涉及肉豆蔻酸对双层膜中泄漏的稳定作用以及由此形成的囊泡转变活化位点。讨论了与天然系统中融合的关系。
