Miura T, Kitaoka M
Arch Virol. 1977;53(4):281-6. doi: 10.1007/BF01315626.
Virus isolation has been attempted from the blood, spleen, liver and kidneys of bats. Nine viruses (1.1 per cent) have been isolated so far from 838 bats collected in different places in Japan. Two of the isolates (TOK-1350 and TOK-1362) were isolated from Vespertilio supernas collected in Fukushima Prefecture. They killed suckling mice within 3-5 days after intracerebral inoculation. The remaining seven isolates were all filtrable through Millipore filters (0.22 micron) and were pathogenic for suckling mice but not adults. One of seven strains (KY-5) was ether resistant and another (TOK-292) was partially ether resistant and both were resistant to sodium deoxycholate (SDC); another (KY-663) was partially resistant to SDC. The sucrose acetone (SA) antigen of each new isolate did not show haemagglutination (HA) with goose red cells, even after protamine or ultrasonic treatment. Complement fixing (CF) activity was found in four of the seven strains. Both TOK-949 and VS-820 were collected at the same place and probably have the same CF activity. The remaining five strains are apparently different. Both YF-17D and Rio Bravo gave high HI titre with antisera against KY-5, TOK-949, TOK-1280, VS-820 and VeJ-822. They thus seemed to be flaviviruses although they were distinct from Japanese encephalitis virus (JEV) strains. From 1 to 10 per cent of bat sera contained antibody against JEV.
已尝试从蝙蝠的血液、脾脏、肝脏和肾脏中分离病毒。到目前为止，从日本不同地方采集的838只蝙蝠中分离出了9种病毒（占1.1%）。其中两种分离株（TOK - 1350和TOK - 1362）是从福岛县采集的日本伏翼中分离出来的。它们在脑内接种后3 - 5天内杀死乳鼠。其余七种分离株均可通过密理博滤器（0.22微米）过滤，对乳鼠有致病性，但对成年鼠无致病性。七种毒株中的一种（KY - 5）对乙醚有抗性，另一种（TOK - 292）对乙醚部分有抗性，且两者均对脱氧胆酸钠（SDC）有抗性；另一种（KY - 663）对SDC部分有抗性。即使经过鱼精蛋白或超声处理，每种新分离株的蔗糖丙酮（SA）抗原也不与鹅红细胞发生血凝（HA）反应。在七种毒株中有四种发现了补体结合（CF）活性。TOK - 949和VS - 820是在同一地点采集的，可能具有相同的CF活性。其余五种毒株明显不同。黄热病毒17D株（YF - 17D）和里约布拉沃病毒（Rio Bravo）与抗KY - 5、TOK - 949、TOK - 1280、VS - 820和VeJ - 822的抗血清产生了高血凝抑制（HI）效价。因此，尽管它们与日本脑炎病毒（JEV）毒株不同，但似乎是黄病毒。1%至10%的蝙蝠血清含有抗JEV抗体。
