Desselberger U
Arch Virol. 1977;53(4):335-49. doi: 10.1007/BF01315632.
The influenza virus strains A/Sing/1/57 (H2N2), A/Bel/42 (H0N1) and A/Bel/42 (HO)-A/Sing/1/57 (N2) were treated with bromelain under reducing conditions and with reducing agent alone, and the antigenicity of the neuraminidase (NA) of intact virus and of the split products was tested comparatively. It was found that the antigenicity of NA was influenced quantitatively and qualitatively by the preparation procedure. Antineuraminidase (AN) antibodies obtained after vaccination of guinea pigs with intact virus and with split products differed in their cross-reactivity with heterologous neuraminidases. In several cases, the quantity of AN antibody formation depended on the hemagglutinin (HA) dose present in the vaccines. The N2 NA on the recombinant virus was significantly more sensitive to treatment with reducing agent than was the N2 NA on the parent virus. AN antibodies directed against N2 NA on the recombinant differed qualitatively from that directed against N2 NA of parent virus. The results warrant the conclusion that the antigenicity of isolated NA or of NA on recombinant virus can differ from that of the NA on intact homologous virus and that such alterations could influence the determination of antigenic relationship between neuraminidases.
将甲型流感病毒株A/新加坡/1/57（H2N2）、A/比利时/42（H0N1）和A/比利时/42（HO）-A/新加坡/1/57（N2）在还原条件下用菠萝蛋白酶处理以及仅用还原剂处理，然后对完整病毒和裂解产物的神经氨酸酶（NA）抗原性进行比较测试。结果发现，NA的抗原性在数量和质量上受制备程序的影响。用完整病毒和裂解产物对豚鼠进行疫苗接种后获得的抗神经氨酸酶（AN）抗体与异源神经氨酸酶的交叉反应性有所不同。在几种情况下，AN抗体形成的量取决于疫苗中存在的血凝素（HA）剂量。重组病毒上的N2 NA比亲本病毒上的N2 NA对还原剂处理明显更敏感。针对重组病毒上N2 NA的AN抗体在质量上与针对亲本病毒N2 NA的AN抗体不同。这些结果证明了以下结论：分离的NA或重组病毒上NA的抗原性可能与完整同源病毒上NA的抗原性不同，并且这种改变可能影响神经氨酸酶之间抗原关系的判定。
