Webster R G, Darlington R W
J Virol. 1969 Aug;4(2):182-7. doi: 10.1128/JVI.4.2.182-187.1969.
Myxoviruses were disrupted with Tween 20 at high pH, and the major surface antigens were separated in biologically active form. The neuraminidase had a sedimentation coefficient of 10.8S, and the hemagglutinin had a sedimentation coefficient of 8.1S. Electron microscopic examination of negatively stained preparations revealed structures identical in size and morphology to the neuraminidase and hemagglutinin subunits described by others. Inhibition of neuraminidase activity by antibody to the hemagglutinin which occurred with intact viruses (probably for "steric" reasons) did not occur after the viruses were disrupted with Tween 20. Serological assays for neuraminidase were possible in the presence of the mild surfactant, whereas serological assays for hemagglutinin were possible after removal of the reagent. Disruption of myxoviruses with Tween 20 therefore provides a method for the independent study of these antigens during antigenic drift.
黏液病毒在高pH值下用吐温20进行裂解，主要表面抗原以生物活性形式分离出来。神经氨酸酶的沉降系数为10.8S，血凝素的沉降系数为8.1S。对经负染处理的制剂进行电子显微镜检查，发现其结构在大小和形态上与其他人描述的神经氨酸酶和血凝素亚基相同。完整病毒存在时血凝素抗体对神经氨酸酶活性的抑制作用（可能是由于“空间位阻”原因）在用吐温20裂解病毒后不再出现。在温和表面活性剂存在的情况下可以进行神经氨酸酶的血清学检测，而血凝素的血清学检测则在去除该试剂后进行。因此，用吐温20裂解黏液病毒为在抗原漂移期间独立研究这些抗原提供了一种方法。
