Miller M L, Dang H, Talal N
Department of Pediatrics, University of Texas Health Science Center, San Antonio 78284-8805.
Clin Exp Immunol. 1990 Oct;82(1):27-32. doi: 10.1111/j.1365-2249.1990.tb05399.x.
We investigated the role of MRl T cells in the induction of anti-Sm antibodies and Y2 idiotype. Four injections of Sm antigen in Freund's complete adjuvant were required to induce peak amounts of specific anti-Sm antibody in young BALB/c and MRL/+ mice. The Y2 idiotype was expressed in MRL/+ mice but not in BALB/c mice. Expression of both anti-Sm, predominantly IgG2a heavy chain, and Y2 idiotype was augmented in MRL/+ mice after two injections of Sm if, prior to immunization, mice received splenic T cells from naive MRL/lpr or immunized, but not naive MRL/+ mice. These results suggest that the lpr gene contributes to the ability of autoimmune T cells to augment the anti-Sm antibody response. Treatment of primed MRL/+ donor T cells with anti-CD4, but not anti-CD8, antibodies and complement removed the ability to augment anti-Sm antibody production. In contrast, augmentation of Y2 idiotype production was abrogated by pretreatment of donor T cells with either anti-CD4 or anti-CD8. These results suggest that, while MRL/+ CD4+ T cells play an important role in anti-Sm antibody production, additional interaction between CD4+ and CD8+ T cells augments Y2 expression.
我们研究了MRL T细胞在诱导抗Sm抗体和Y2独特型中的作用。在年轻的BALB/c和MRL/+小鼠中，需要在弗氏完全佐剂中注射四次Sm抗原才能诱导出最高量的特异性抗Sm抗体。Y2独特型在MRL/+小鼠中表达，但在BALB/c小鼠中不表达。如果在免疫前，小鼠接受来自未免疫的MRL/lpr或已免疫但非未免疫的MRL/+小鼠的脾T细胞，那么在MRL/+小鼠中，注射两次Sm后，抗Sm（主要是IgG2a重链）和Y2独特型的表达都会增强。这些结果表明，lpr基因有助于自身免疫性T细胞增强抗Sm抗体反应的能力。用抗CD4抗体而非抗CD8抗体及补体处理已致敏的MRL/+供体T细胞，会消除其增强抗Sm抗体产生的能力。相反，用抗CD4或抗CD8预处理供体T细胞会消除Y2独特型产生的增强作用。这些结果表明，虽然MRL/+ CD4+ T细胞在抗Sm抗体产生中起重要作用，但CD4+和CD8+ T细胞之间的额外相互作用会增强Y2的表达。
