Kleinman R E, Harmatz P R, Jacobson L A, Udall J N, Bloch K J, Walker W A
Pediatr Res. 1983 Jun;17(6):449-51. doi: 10.1203/00006450-198306000-00003.
Female New Zealand white rabbits were immunized with bovine serum albumin (BSA). Litters which had never suckled, from immunized and nonimmunized rabbits, were tested at 4 h, 24 h, and 48 h after birth. After obtaining an initial blood sample, all infant rabbits were gavaged with 100 mg of BSA plus tracer amounts of [125I]-BSA. Infant rabbits born to immunized mothers had circulating antibody before feeding and pups from nonimmunized mothers had no detectable antibody to BSA. The fed animals were sacrificed at 3-4 h after gavage. Serum obtained from cardiac and portal blood was examined for protein bound radioactivity and for the presence of immunoreactive BSA (iBSA) by electroimmunodiffusion. All infant rabbits had radioactivity in their blood. Approximately 50% of the radioactivity in the serum of infant rabbits from nonimmunized does was protein bound and all of these animals had iBSA in portal or cardiac serum samples. Of the 33 infant rabbits from immunized does, only four had protein bound radioactivity in their serum. This radioactivity appeared to be associated with circulating immune complexes of [125I]-BSA-rabbit-anti-BSA antibodies. None of the 33 infant rabbits had iBSA detectable by electroimmunodiffusion.
将雌性新西兰白兔用牛血清白蛋白（BSA）进行免疫。从未哺乳的免疫兔和未免疫兔的幼崽在出生后4小时、24小时和48小时进行检测。采集初始血样后，所有幼兔经口灌胃给予100毫克BSA加微量示踪剂[125I]-BSA。免疫母兔所生的幼兔在喂食前就有循环抗体，而未免疫母兔所生的幼崽未检测到针对BSA的抗体。灌胃后3 - 4小时处死喂食后的动物。检测心脏血和门静脉血血清中的蛋白结合放射性，并通过电免疫扩散法检测免疫反应性BSA（iBSA）的存在。所有幼兔血液中均有放射性。未免疫母兔所生幼兔血清中约50%的放射性与蛋白结合，所有这些动物的门静脉或心脏血清样本中均有iBSA。在33只免疫母兔所生的幼兔中，只有4只血清中有蛋白结合放射性。这种放射性似乎与[125I]-BSA - 兔抗BSA抗体的循环免疫复合物有关。33只幼兔中没有一只通过电免疫扩散法检测到iBSA。
