[Optimization of the medium for biotin biosynthesis by a culture of Rhizopus delemar].

The effect of nitrogen and carbon sources on the biotin biosynthesis by the fungal culture Rhizopus delemar 1428 has been investigated. Peptone and soy-bean flour have been shown to stimulate biotin synthesis. With the aid of mathematical planning methods the optimal composition of the nutrient medium and optimal concentrations of its components have been selected. The cultivation of Rh. delemar 1428 on the thus developed medium leads to the accumulation of 600 micrograms/1 biotin in the culture filtrate.