Ribári O, Sziklai I
Acta Otolaryngol. 1983 May-Jun;95(5-6):580-4. doi: 10.3109/00016488309139446.
We have differentially precipitated 2 fractions of noncollagen proteins extracted from separately pooled superstructure and footplate of surgically removed stapes of patients from both sexes, who have suffered from otosclerosis. The two fractions were: EDTA-soluble, CaCl2 precipitable (Fraction-1) and EDTA-soluble, CaCl2 non-precipitable proteins. The protein pattern of these two fractions was compared by isoelectric focusing and two-dimensional gel electrophoresis. The phosphoprotein fraction (Fraction-1) of the otosclerotic stapes footplate contained acidic (pI 3-6), low molecular weight (20-40 kD) proteins, which were not detected in the superstructure and temporal cortical bone. Two-dimensional mapping of the Fraction-2 showed predominantly acidic proteins in the footplate and some basic minor components in the superstructure. The lack of the low molecular weight proteins in the superstructure proves the localisation of the otosclerotic process only in the footplate.
我们对从患有耳硬化症的男女患者手术切除的镫骨上层结构和足板中分别提取的非胶原蛋白进行了差异沉淀，得到了两个组分。这两个组分分别是：EDTA 可溶、CaCl₂ 可沉淀的组分（组分 1）和 EDTA 可溶、CaCl₂ 不可沉淀的蛋白质。通过等电聚焦和二维凝胶电泳对这两个组分的蛋白质图谱进行了比较。耳硬化镫骨足板的磷蛋白组分（组分 1）含有酸性（pI 3 - 6）、低分子量（20 - 40 kD）的蛋白质，这些蛋白质在上层结构和颞骨皮质骨中未检测到。组分 2 的二维图谱显示，足板中主要是酸性蛋白质，上层结构中有一些碱性次要成分。上层结构中缺乏低分子量蛋白质，证明耳硬化过程仅局限于足板。
