Araki M, Sato F, Saito T
Arch Histol Jpn. 1983 Apr;46(2):191-201. doi: 10.1679/aohc.46.191.
Dissociated cerebella of newborn rats were cultured in vitro as a monolayer for a long period, and ependymal cell differentiation was investigated by scanning and transmission electron microscopy. Cells with actively beating cilia were recognized on the 3rd day, and gradually increased in number thereafter. At nine months of culturing, a number of areas appeared, some of which consisted of several hundreds of ciliated cells. Cultured ependymal cells showed a striking resemblance in their fine structure to the intact ependyma. They were always found to grow upon the feltwork of astrocytic processes which served as a subependymal structure, as observed in the intact ependyma. It is possible that astrocytes may play some role in ependymal differentiation. Cultured ependymal cells are helpful in understanding the control mechanism for the metachronal cilial movement.
将新生大鼠的离体小脑单层培养较长时间，并用扫描电子显微镜和透射电子显微镜研究室管膜细胞的分化。在第3天识别出具有活跃摆动纤毛的细胞，此后数量逐渐增加。培养9个月时，出现了许多区域，其中一些区域由数百个纤毛细胞组成。培养的室管膜细胞在精细结构上与完整的室管膜惊人地相似。正如在完整室管膜中观察到的那样，它们总是生长在作为室管膜下结构的星形胶质细胞突起网络上。星形胶质细胞可能在室管膜分化中起某种作用。培养的室管膜细胞有助于理解纤毛同步运动的控制机制。
