Rieke G K, Jordan F L, Wynder H J, Thomas W E
Department of Anatomy, Meharry Medical College, Nashville, TN 37208.
J Neurosci Res. 1987;18(3):484-92. doi: 10.1002/jnr.490180316.
Ultrastructural features of ependymal cells growing in primary cultures of dissociated cerebral cortical tissue were investigated using electron microscopy. The ependyma exhibited a specific orientation in tissue culture such that the cell surface corresponding to the apical surface in situ was directed toward the culture medium. The membrane on the apical surface of cultured ependyma was characterized by the presence of cilia and microvilli. The lateral margins were marked by a variety of junctions: zonula occludens, zonula adherens, and membrane interdigitations. The cell cytoplasm contained pleomorphic mitochondria, Golgi profiles, clusters of ribosomes, rough endoplasmic reticulum, and particularly profuse scattered microfilaments and microtubules. These features support the identity of the cells as ependymal in the cultures and establish them as a relatively accurate reflection of ependyma in situ.
利用电子显微镜研究了在离体大脑皮质组织原代培养物中生长的室管膜细胞的超微结构特征。室管膜在组织培养中呈现出特定的取向，使得在原位对应于顶端表面的细胞表面朝向培养基。培养的室管膜顶端表面的膜以存在纤毛和微绒毛为特征。侧缘有多种连接结构：紧密连接、黏着连接和膜交错。细胞质中含有多形性线粒体、高尔基体、核糖体簇、粗面内质网，特别是大量散在的微丝和微管。这些特征支持培养物中的细胞为室管膜细胞，并表明它们是原位室管膜的相对准确反映。
