Wong P Y, Tsang A Y
Biochem Biophys Res Commun. 1983 Jul 29;114(2):653-6. doi: 10.1016/0006-291x(83)90830-6.
Radioimmunoassay (RIA) and radioreceptor assay (RRA) for the 32K Rat Epididymal Protein (REP) have been developed. Washed intact rat epididymal spermatozoa were used as receptors in the RRA. The effects of epididymal and seminal fluids of other species in these assays were studied. The results showed that there is species specificity in the immunoreactivity and receptor binding activity of sperm coating proteins.
已开发出针对32K大鼠附睾蛋白(REP)的放射免疫测定法(RIA)和放射受体测定法(RRA)。在RRA中,使用经洗涤的完整大鼠附睾精子作为受体。研究了这些测定法中其他物种的附睾液和精液的作用。结果表明,精子包被蛋白的免疫反应性和受体结合活性存在物种特异性。