Shaikh N A, Kuksis A
Can J Biochem Cell Biol. 1983 Jun;61(6):370-7. doi: 10.1139/o83-051.
The effect of fat absorption on the phospholipid turnover of rat intestinal mucosa was determined in animals receiving single fatty meals by stomach tube or multiple meals in the form of corn-oil-soaked laboratory chow diet. The specific activity and relative specific activity of the total phospholipids and of individual phospholipid classes were measured in the isolated jejunal villus cells of fasting and fat-fed animals following an injection of radioactive inorganic phosphate 0.5-31 h prior to sacrifice, which was scheduled to coincide with the peak of fat absorption (2.5-3 h after the last meal). It was shown that the relative specific activity of the fat-absorbing cells increased by about 33% when the samples were taken 0.5 h after intravenous injection of radioactive phosphate. Samples taken 11 and 31 h after the introduction of the radioactive phosphate showed about 16% decrease in the relative specific activity of the phospholipids of the fat-absorbing cells when compared with the fasting controls. These changes in the relative specific activity of the total phospholipids included all phospholipid classes and corresponded to the recently described expansion of the cellular phospholipid pool owing partly to increased de novo synthesis of the membrane phospholipids. The present results are consistent with the known biochemical and physiological changes taking place in the mucosal cells during fat absorption and transport and find support in various less direct biochemical and morphometric measurements.
通过胃管给大鼠单次喂食脂肪餐或给予玉米油浸泡的实验室饲料形式的多次喂食，来测定脂肪吸收对大鼠肠黏膜磷脂周转的影响。在处死动物前0.5 - 31小时注射放射性无机磷酸盐，处死时间安排在脂肪吸收高峰期（最后一餐2.5 - 3小时后），然后测定禁食和喂食脂肪动物的分离空肠绒毛细胞中总磷脂及各磷脂类别的比活性和相对比活性。结果显示，静脉注射放射性磷酸盐后0.5小时取样时，脂肪吸收细胞的相对比活性增加约33%。与禁食对照组相比，引入放射性磷酸盐后11小时和31小时取样时，脂肪吸收细胞磷脂的相对比活性降低约16%。总磷脂相对比活性的这些变化包括所有磷脂类别，这与最近描述的细胞磷脂池扩大相符，部分原因是膜磷脂从头合成增加。目前的结果与脂肪吸收和运输过程中黏膜细胞发生的已知生化和生理变化一致，并在各种不太直接的生化和形态测量中得到支持。
