Bidani A K, Churchill P C
Can J Physiol Pharmacol. 1983 Jun;61(6):567-71. doi: 10.1139/y83-087.
The effect of aminophylline (theophylline complexed with ethylenediamine) on the severity of glycerol-induced myohemoglobinuric acute renal failure was examined in rats. In the first series of experiments, one group of rats received twice daily injections of aminophylline following the induction of acute renal failure, and a second group (controls) received twice daily injections of saline. Only one of the aminophylline-injected rats but five of the saline-injected rats died during the 3-day follow-up period. Moreover, mean serum creatinine was lower in the aminophylline-injected rats than in the saline-injected controls on each of the 3 days, demonstrating that aminophylline reduced the renal functional impairment. In the second series, single injections of aminophylline were given at the time of glycerol injections or 3, 6, or 24 h later. As assessed by mean serum creatinine during the 3-day follow up, even single injections had protective effects if given during the initiation phase (0-3 h after glycerol). Since aminophylline dissociates into theophylline in biological fluids, and since theophylline is an adenosine-receptor antagonist, these observations are consistent with the hypothesis that adenosine plays a pathogenic role in myohemoglobinuric acute renal failure in rats.
在大鼠中研究了氨茶碱（与乙二胺络合的茶碱）对甘油诱导的肌红蛋白尿性急性肾衰竭严重程度的影响。在第一系列实验中，一组大鼠在急性肾衰竭诱导后每天接受两次氨茶碱注射，另一组（对照组）每天接受两次生理盐水注射。在3天的随访期内，注射氨茶碱的大鼠中只有1只死亡，而注射生理盐水的大鼠中有5只死亡。此外，在3天中的每一天，注射氨茶碱的大鼠的平均血清肌酐均低于注射生理盐水的对照组，表明氨茶碱减轻了肾功能损害。在第二系列实验中，在注射甘油时或3、6或24小时后单次注射氨茶碱。通过3天随访期间的平均血清肌酐评估，即使在起始阶段（甘油注射后0 - 3小时）单次注射也具有保护作用。由于氨茶碱在生物体液中会解离成茶碱，且茶碱是一种腺苷受体拮抗剂，这些观察结果与腺苷在大鼠肌红蛋白尿性急性肾衰竭中起致病作用的假说一致。
