Sridhar K S, Plasse T F, Holland J F, Shapiro M, Ohnuma T
Cancer Res. 1983 Oct;43(10):4629-31.
Cloning efficiencies of 67 fresh human tumor specimens, consisting of 29 ovarian, 10 lung, and 7 each of colon, breast, mesenchymal, and miscellaneous tumors, from 58 patients were studied using the technique of Hamburger and Salmon to evaluate the effect of incubation in a 5% rather than 20% oxygen environment. Under the low-oxygen tension, carcinomas exhibited an average of 170% increase in cloning efficiency (p less than 0.01). The number of carcinomas forming at least 30 colonies/dish increased from 22 to 27. Mesenchymal tumors, however, exhibited a 20% decrease in cloning efficiency (not significant). A mixture of 5% oxygen, 5% carbon dioxide, and 90% nitrogen gives a higher cloning efficiency than 20% oxygen, 5% carbon dioxide, and 75% nitrogen for certain human carcinomas in semisolid agar.
采用汉堡和萨尔蒙技术，对来自58名患者的67份新鲜人类肿瘤标本（包括29份卵巢肿瘤、10份肺肿瘤以及各7份结肠、乳腺、间充质和其他肿瘤）的克隆效率进行了研究，以评估在5%而非20%氧气环境中孵育的效果。在低氧张力下，癌组织的克隆效率平均提高了170%（p<0.01）。每皿形成至少30个集落的癌组织数量从22个增加到27个。然而，间充质肿瘤的克隆效率降低了20%（无统计学意义）。对于半固体琼脂中的某些人类癌组织，5%氧气、5%二氧化碳和90%氮气的混合气体比20%氧气、5%二氧化碳和75%氮气具有更高的克隆效率。
