Ludwig C, Ludwig R, Obrecht J P
Schweiz Med Wochenschr. 1981 Sep 5;111(36):1313-8.
Hamburger and Salmon have recently developed an in vitro assay for human tumor stem cells which permits formation of colonies of human solid tumors and lymphomas in soft agar. In 50 patients with solid tumors or lymphomas studied by us, tumor colonies grew from effusions and biopsies in 75% of patients (17 of 21) with histologically or cytologically involved specimens. Lymphoid colony growth was obtained in about 25% of specimens (5 of 19) including malignant lymphomas. Conversely, no colony growth was observed in 10 cases where the biopsies or effusions were histologically or cytologically negative. The effect of various conditioned media on tumor growth in agar was investigated. Colony formation of solid tumors was promoted to an especially marked extent by cell-free malignant effusions. This assay yields satisfactory results in solid tumors and appears useful for investigation of different cytotoxic effects of anticancer drugs on the survival of human tumor colony-forming cells.
汉堡和萨尔蒙最近开发了一种针对人类肿瘤干细胞的体外检测方法，该方法能使人类实体瘤和淋巴瘤在软琼脂中形成集落。在我们研究的50例实体瘤或淋巴瘤患者中，75%（21例中的17例）组织学或细胞学检查有病变标本的患者，其肿瘤集落从积液和活检组织中生长出来。在约25%的标本（19例中的5例）包括恶性淋巴瘤中获得了淋巴样集落生长。相反，在10例活检或积液组织学或细胞学检查为阴性的病例中未观察到集落生长。研究了各种条件培养基对琼脂中肿瘤生长的影响。无细胞恶性积液对实体瘤集落形成有特别显著的促进作用。该检测方法在实体瘤中产生了令人满意的结果，似乎有助于研究抗癌药物对人类肿瘤集落形成细胞存活的不同细胞毒性作用。
