Brentani M M, Nagai M A
Cancer Detect Prev. 1983;6(1-2):241-7.
Analysis of estrogen receptor (ER), progesterone receptor (PR), and total activity of lactate dehydrogenase (LDH) as well as electrophoretic separation of the LDH isoenzymes was performed in samples of primary infiltrating ductal carcinomas and normal breast. Estrogen receptor and progesterone receptor were determined by a dextran charcoal procedure. Total LDH activity was assayed in the cytosol by measuring the decrease in NADH absorbance with pyruvate as substrate. The LDH activity was expressed as mumoles cofactor altered per minute per mg DNA. Incidence of ER + PR + tumors was higher in postmenopausal than in premenopausal women but PR + was found in about 70% of ER + cases in both groups. In postmenopausal women the mean level of ER or PR decreased in tumors that had either ER or PR. In premenopausal women differences were not significant, but frequency of PR + depended on the phase of the menstrual cycle. The mean value of LDH in ER + PR + tumors was lower than in the ER- PR- subset regardless of menopausal status. The mean enzyme level is twofold greater than in normal breast tissue. A large increase in LDH-5 and a small but significant increase in LDH-4 was demonstrated by cellulose acetate electrophoresis.
对原发性浸润性导管癌和正常乳腺样本进行了雌激素受体（ER）、孕激素受体（PR）、乳酸脱氢酶（LDH）总活性分析以及LDH同工酶的电泳分离。雌激素受体和孕激素受体通过葡聚糖活性炭法测定。通过以丙酮酸为底物测量NADH吸光度的降低来测定细胞质中的总LDH活性。LDH活性表示为每毫克DNA每分钟改变的微摩尔辅因子数。绝经后女性中ER + PR +肿瘤的发生率高于绝经前女性，但两组中约70%的ER +病例中发现PR +。在绝经后女性中，具有ER或PR的肿瘤中ER或PR的平均水平降低。在绝经前女性中差异不显著，但PR +的频率取决于月经周期阶段。无论绝经状态如何，ER + PR +肿瘤中LDH的平均值均低于ER - PR -亚组。平均酶水平比正常乳腺组织高两倍。醋酸纤维素电泳显示LDH - 5大幅增加，LDH - 4有小幅但显著增加。
