Jones R, Dennison M E, Burnstock G
Cell Tissue Res. 1983;232(2):265-79. doi: 10.1007/BF00213786.
The physiological, pharmacological and morphological characteristics of guinea-pig vas deferens supplied by hypogastric nerves rendered inactive by decentralisation were compared with those of vas deferens in which the nerve supply had been chronically stimulated for 3-9 days using implanted electrodes. No change was seen in decentralised preparations prior to 7 days, but from 8-15 days, increased sensitivity to application of noradrenaline in vitro was observed, which was shown to be related to reduced transmitter uptake by nerve terminals as well as to an increase in postjunctional sensitivity; there was also increased fatigability 7-14 days following decentralisation. Continuous stimulation of hypogastric nerves at 2 Hz for 4-8 h daily for 4-8 days resulted in enhanced transmitter uptake and reduced responses to noradrenaline; this was associated with a slight increase in noradrenaline content and a faster adrenergic neuromuscular response with a shorter latency. No appreciable changes in nerve or muscle structure studied by electron microscopy were observed following decentralisation, but there was an increase of between 12.5 and 29.6% in the number of close (less than 100 nm) neuromuscular junctions following chronic stimulation for 8 days.
将去神经支配（使其失去活性）的豚鼠输精管的生理、药理和形态学特征，与使用植入电极对神经支配进行3 - 9天慢性刺激后的输精管特征进行了比较。在7天之前，去神经支配的标本未观察到变化，但在8 - 15天，观察到体外对去甲肾上腺素应用的敏感性增加，这被证明与神经末梢递质摄取减少以及接头后敏感性增加有关；去神经支配后7 - 14天疲劳性也增加。每天以2 Hz持续刺激腹下神经4 - 8小时，持续4 - 8天，导致递质摄取增强，对去甲肾上腺素的反应降低；这与去甲肾上腺素含量略有增加以及肾上腺素能神经肌肉反应更快、潜伏期更短有关。去神经支配后，通过电子显微镜研究未观察到神经或肌肉结构有明显变化，但在慢性刺激8天后，紧密（小于100 nm）神经肌肉接头的数量增加了12.5%至29.6%。
