Jones R, Yokota R, Burnstock G
Cell Tissue Res. 1983;232(2):281-93. doi: 10.1007/BF00213787.
Previous studies have shown that minced regenerating smooth muscle of the guinea-pig vas deferens becomes reinnervated by nerves growing in from the surrounding intact vas deferens. Using electron microscopy, we have examined the effect of altering activity in the preganglionic nerves, either by decentralisation, or by chronic stimulation of the hypogastric nerve, in vivo, on the reinnervation of regenerating smooth muscle cells. Chronic stimulation induced earlier reinnervation than that seen in unstimulated (sham-operated) or decentralised preparations; the number of nerve profiles present in four preparations stimulated for up to 7 days was approximately 10-20 times that seen in unstimulated or decentralised preparations. However, electron micrographs revealed that "empty" nerve terminals were a feature following stimulation for longer periods. Decentralised preparations showed little change of reinnervation, at least up to 7 weeks. Compensatory changes in the density of innervation were found in the unstimulated contralateral vas deferens.
先前的研究表明，豚鼠输精管再生的平滑肌碎块会被从周围完整输精管生长进来的神经重新支配。我们利用电子显微镜，在体内研究了通过去神经支配或慢性刺激腹下神经来改变节前神经活动，对再生平滑肌细胞重新神经支配的影响。慢性刺激比未刺激（假手术）或去神经支配的标本更早诱导重新神经支配；在长达7天受到刺激的4个标本中，神经断面数量大约是未刺激或去神经支配标本的10 - 20倍。然而，电子显微镜照片显示，长时间刺激后，“空”神经末梢是一个特征。去神经支配的标本至少在7周内重新神经支配几乎没有变化。在未刺激的对侧输精管中发现了神经支配密度的代偿性变化。
