Snyder J, Hymer W C, Wilfinger W W
Cell Tissue Res. 1978 Aug 16;191(3):379-88. doi: 10.1007/BF00219803.
Fragments of pituitary tissue obtained from a total of 37 patients with either breast cancer, diabetic retinopathy, galactorrhea, or acromegaly were dissociated into single cell suspensions prior to cell culture. Release of human growth hormone (hGH) and human prolactin (hPRL) into the culture medium was measured by radioimmunoassay. During a 3-week culture period, prolactin cells released 9--13 times the intracellular levels of hPRL at the time of seeding, whereas hGH release from growth hormone cells was only 1--2 times that of their initial intracellular level during this same time. Both growth hormone and prolactin cells retained distinctive ultrastructural features during culture. The prolactin cells responded to TRH stimulation by elevated release of PRL into the medium. No evidence for mitotic division of prolactin cells in vitro was found.
从总共37例患有乳腺癌、糖尿病性视网膜病变、溢乳症或肢端肥大症的患者身上获取垂体组织碎片，在进行细胞培养之前将其解离成单细胞悬液。通过放射免疫测定法测量人生长激素（hGH）和人催乳素（hPRL）释放到培养基中的量。在为期3周的培养期内，催乳素细胞释放的hPRL量是接种时细胞内水平的9至13倍，而生长激素细胞释放的hGH在此期间仅为其初始细胞内水平的1至2倍。生长激素细胞和催乳素细胞在培养过程中均保留了独特的超微结构特征。催乳素细胞对促甲状腺激素释放激素（TRH）刺激的反应是向培养基中释放更多的PRL。未发现体外催乳素细胞有丝分裂的证据。
